WP1
MXene Synthesis, Functionalization, and Characterization
The primary objective of WP1 is to design, synthesize, functionalize, and comprehensively characterize MXene-based nanomaterials tailored for antibacterial and regenerative wound healing applications. This work package will deliver a robust foundation for subsequent scaffold fabrication and biological validation by establishing:
- Scalable, reproducible MXene synthesis protocols capable of producing high-yield, phase-pure Ti- and Nb-based MXenes with controlled flake size, minimal cytotoxic terminations (–F, –Cl), and enhanced colloidal stability suitable for biomedical use.
- Innovative surface functionalization strategies, including PDA coating for biocompatible and adhesive interfaces, followed by covalent and non-covalent conjugation of therapeutic payloads such as MRSA-targeting antibodies and MSC-derived secretome, preserving bioactivity and ensuring controlled release.
- Advanced, multiscale characterization workflows combining structural (XRD, SEM/TEM), chemical (XPS, FTIR, Raman), and optical/electrical analyses with cutting-edge ultrafast photothermal measurements (TAS/TRTS) to quantify MXene stability, conductivity, and NIR-induced thermal response. Emphasis will be placed on reproducibility, quality control, and batch-to-batch consistency to de-risk downstream translational steps.
By integrating scalable production, smart biofunctionalization, and state-of-the-art characterization, WP1 will generate clinically relevant, multifunctional MXenes ready for incorporation into electrospun wound-healing scaffolds and subsequent preclinical validation.